Journal: International journal of molecular sciences

Article Title: Surgical Site-Released Tissue Is Potent to Generate Bone onto TCP and PCL-TCP Scaffolds In Vitro.

doi: 10.3390/ijms242115877

Figure Lengend Snippet: Figure 6. (a–c) ELISA boxplots of the osteogenic markers osteocalcin (a), osteopontin (b), and collagen I (c). Boxplots compare the concentration of ocn, opn, and col I in the control group with the PCL-TCP and β-TCP group over the cultivation time of 6 weeks. Groups are further subdivided into the condition’s agarose and glue, to examine if the chosen adhesive, used for the attachment of the scaffolds onto the plastic wells, makes a difference in protein expression. The boxblots indicating the median within the 25–75% percentile. The dots represent single outliners during measurements.

Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) (human Duoset ELISA kit, R&D Systems, Bio Techne, Minneapolis, MN, USA) were then performed using the supernatants at a dilution of 1:10 for osteocalcin (1:50; pure for collagen and pure for osteopontin) according to the manufacturer’s protocol and measured at 450 nm using a Multiscan Ascent reader (Thermo Scientific).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Control, Adhesive, Expressing

Journal: Biomacromolecules

Article Title: Cytosolic Delivery Mediated via Electrostatic Surface Binding of Protein, Virus, or siRNA Cargos to pH-Responsive Core−Shell Gel Particles

doi: 10.1021/bm801199z

Figure Lengend Snippet: Figure 3. Cytosolic delivery of ova for OT-I T cell priming. (A) BMDCs were incubated with different concentrations of soluble ova (O), ova- coated PDEAEMA CSPs (2), or ova-coated PMMA CSPs (9) in complete medium containing 10% FBS, before being washed and mixed with naı¨ve OT-1 ova-specific CD8+ T-cells. The concentration of particles was fixed at 25 µg/mL for all ova doses. IFN-γ secreted by the T-cells in response to antigen presentation by the DCs was measured by ELISA after 3 days. Shown are mean ( SD of triplicate samples. (B) BMDCs were pulsed with ova in different forms: soluble ova (10 µg/mL) for 1 h or during an entire 3 day coculture, ova mixed with PDEAEMA or PMMA CSPs (without prebinding to particles, ova + CSPs), or ova adsorbed to PDEAEMA or PMMA CSPs prior to addition to DCs (ova-CSPs) in complete medium containing 10% FBS. DCs were washed following antigen pulsing and then cocultured with OT-I T-cells. IFN-γ secreted by T-cells in response to antigen presentation by the DCs was measured by ELISA after 3 days. Shown are mean ( SD of triplicate samples.

Article Snippet: Interferon gamma (IFN-γ) in the supernatants of these cultures was then quantified using an ELISA kit (murine Interferon gamma (IFNγ) Duoset ELISA Development Kit, R&D Systems) according to the manufacturer’s instructions.

Techniques: Incubation, Concentration Assay, Immunopeptidomics, Enzyme-linked Immunosorbent Assay